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axio observer z1 inverted microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axio observer z1 inverted microscope
    Axio Observer Z1 Inverted Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 7752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer/Primovert+Compact+Inverted+Microscope/pm42919372-72-50-48
    Average 99 stars, based on 7752 article reviews
    axio observer z1 inverted microscope - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Absence of Microglial Activation and Maintained Hippocampal Neurogenesis in a Transgenic Mouse Model of Crohn’s Disease
    Article Snippet: .. TUNEL + apoptotic cells were quantified in 3–4 brain sections per animal and in 3 z stacks with 1 μm intervals recorded per section under a 20× objective using a LSM780 confocal laser scanning microscope with an inverted Axio Observer (Zeiss, Oberkochen, Germany). ..

    Article Title: Absence of Microglial Activation and Maintained Hippocampal Neurogenesis in a Transgenic Mouse Model of Crohn's Disease.
    Article Snippet: .. TUNEL+ apoptotic cells were quantified in 3–4 brain sections per animal and in 3 z stacks with 1 μm intervals recorded per section under a 20× objective using a LSM780 confocal laser scanning microscope with an inverted Axio Observer (Zeiss, Oberkochen, Germany). ..

    Laser-Scanning Microscopy:

    Article Title: Absence of Microglial Activation and Maintained Hippocampal Neurogenesis in a Transgenic Mouse Model of Crohn’s Disease
    Article Snippet: .. TUNEL + apoptotic cells were quantified in 3–4 brain sections per animal and in 3 z stacks with 1 μm intervals recorded per section under a 20× objective using a LSM780 confocal laser scanning microscope with an inverted Axio Observer (Zeiss, Oberkochen, Germany). ..

    Article Title: Absence of Microglial Activation and Maintained Hippocampal Neurogenesis in a Transgenic Mouse Model of Crohn's Disease.
    Article Snippet: .. TUNEL+ apoptotic cells were quantified in 3–4 brain sections per animal and in 3 z stacks with 1 μm intervals recorded per section under a 20× objective using a LSM780 confocal laser scanning microscope with an inverted Axio Observer (Zeiss, Oberkochen, Germany). ..

    Article Title: Absence of Microglial Activation and Maintained Hippocampal Neurogenesis in a Transgenic Mouse Model of Crohn's Disease.
    Article Snippet: Z stacks with 2 μm interval and a total thickness of 20 μm of sections co-stained for Iba1, Mcm2, and CD68 were acquired with an Axio Observer Z1 inverted fluorescence microscope with an ApoTome (Zeiss, Oberkochen, Germany) under a 40× objective. .. To determine the total numbers of Mcm2+-proliferating cells in the subgranular zone of the DG and the numbers of DCX+ immature neurons, tile scans of confocal z stacks with 1 μm intervals and a total thickness of 20 μm were acquired at an LSM780 confocal laser scanning microscope with an inverted Axio Observer (Zeiss, Oberkochen, Germany) under a 20× objective, covering the whole DG on every brain section. ..

    Fluorescence:

    Article Title: Strategic ultrasonic degradation enhances immunomodulatory and hypolipidemic activities of Hericium Erinaceus β-glucan.
    Article Snippet: Bioactive β-glucans from Hericium erinaceus possess health benefits, yet the structure-function relationships of these polysaccharides remain incompletely elucidated.. This study employed an ultrasonic degradation strategy to modify a high-molecular-weight β-glucan (H6PC20, 2.39 × 106 g/mol) from its fruiting bodies.. Ultrasonic treatment yielded three degraded fractions (H6PC20 A-C) with molecular weights ranging from 4.60 × 105 g/ mol to 1.84 × 105 g/mol, accompanied by a sharp reduction in intrinsic viscosity from 1231.99 mL/g to 108.76 mL/g.

    Microscopy:

    Article Title: Strategic ultrasonic degradation enhances immunomodulatory and hypolipidemic activities of Hericium Erinaceus β-glucan.
    Article Snippet: Bioactive β-glucans from Hericium erinaceus possess health benefits, yet the structure-function relationships of these polysaccharides remain incompletely elucidated.. This study employed an ultrasonic degradation strategy to modify a high-molecular-weight β-glucan (H6PC20, 2.39 × 106 g/mol) from its fruiting bodies.. Ultrasonic treatment yielded three degraded fractions (H6PC20 A-C) with molecular weights ranging from 4.60 × 105 g/ mol to 1.84 × 105 g/mol, accompanied by a sharp reduction in intrinsic viscosity from 1231.99 mL/g to 108.76 mL/g.

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Article Title: In vitro models to mimic tumor endothelial cell-mediated immune cell reprogramming in lung adenocarcinoma.
    Article Snippet: 311 Following hydration (Akoya Biosciences) step, tissue section was blocked in 312 Staining buffer (Akoya Biosciences) at room temperature for 20 minutes and 313 stained with the 6 plex PhenoCycler antibody panel in a humidity chamber 3 hours 314 at room temperature. .. 324 PhenoCycler imaging was performed with an Axio Observer (Zeiss) inverted 325 microscope equipped with an ORCA Flash 4.0 LT camera (Hamamatsu). .. After incubation with antibodies, tissue section was washed twice 317 and post-fixated with 1.6% PFA (Sigma) Storage buffer (Akoya Biosciences).

    Liposomes:

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Incubation:

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Purification:

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Incubator:

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Light Microscopy:

    Article Title: CANDiT: A machine learning framework for differentiation therapy in colorectal cancer
    Article Snippet: QuantStudio5 , Applied Biosystems , Cat# A28140 RRID: SCR_020240. .. Light Microscope (brightfield images) , Carl Zeiss LLC , Axio Observer, Inverted; 491917-0001-000. .. Fisherbrand 150 Handheld Homogenizer , Fisher Scientific , Cat# 15340168.

    Brightfield:

    Article Title: CANDiT: A machine learning framework for differentiation therapy in colorectal cancer
    Article Snippet: QuantStudio5 , Applied Biosystems , Cat# A28140 RRID: SCR_020240. .. Light Microscope (brightfield images) , Carl Zeiss LLC , Axio Observer, Inverted; 491917-0001-000. .. Fisherbrand 150 Handheld Homogenizer , Fisher Scientific , Cat# 15340168.

    Imaging:

    Article Title: In vitro models to mimic tumor endothelial cell-mediated immune cell reprogramming in lung adenocarcinoma.
    Article Snippet: 311 Following hydration (Akoya Biosciences) step, tissue section was blocked in 312 Staining buffer (Akoya Biosciences) at room temperature for 20 minutes and 313 stained with the 6 plex PhenoCycler antibody panel in a humidity chamber 3 hours 314 at room temperature. .. 324 PhenoCycler imaging was performed with an Axio Observer (Zeiss) inverted 325 microscope equipped with an ORCA Flash 4.0 LT camera (Hamamatsu). .. After incubation with antibodies, tissue section was washed twice 317 and post-fixated with 1.6% PFA (Sigma) Storage buffer (Akoya Biosciences).

    Article Title: In vitro models to mimic tumor endothelial cell-mediated immune cell reprogramming in lung adenocarcinoma
    Article Snippet: Following washing, tissue section was fixated in final fixative solution (Akoya Biosciences) for 20 min at room temperature. .. PhenoCycler imaging was performed with an Axio Observer (Zeiss) inverted microscope equipped with an ORCA Flash 4.0 LT camera (Hamamatsu). .. PhenoCycler imaging was performed with an Axio Observer (Zeiss) inverted microscope equipped with an ORCA Flash 4.0 LT camera (Hamamatsu).

    Inverted Microscopy:

    Article Title: In vitro models to mimic tumor endothelial cell-mediated immune cell reprogramming in lung adenocarcinoma
    Article Snippet: Following washing, tissue section was fixated in final fixative solution (Akoya Biosciences) for 20 min at room temperature. .. PhenoCycler imaging was performed with an Axio Observer (Zeiss) inverted microscope equipped with an ORCA Flash 4.0 LT camera (Hamamatsu). .. PhenoCycler imaging was performed with an Axio Observer (Zeiss) inverted microscope equipped with an ORCA Flash 4.0 LT camera (Hamamatsu).



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